Flavonoid 3',5'-hydroxylase

Template:Infobox enzyme Flavonoid 3',5'-hydroxylase (Template:EC number was wrongly classified as Template:EC number in the past) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction

flavanone + 2 NADPH + 2 H⁺ + 2 O₂ $\rightleftharpoons$ 3',5'-dihydroxyflavanone + 2 NADP⁺ + 2 H₂O (overall reaction)

(1a) flavanone + NADPH + H⁺ + O₂ $\rightleftharpoons$ 3'-hydroxyflavanone + NADP⁺ + H₂O

(1b) 3'-hydroxyflavanone + NADPH + H⁺ + O₂ $\rightleftharpoons$ 3',5'-dihydroxyflavanone + NADP⁺ + H₂O

Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).

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