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...um (phenol red free DMEM with charcoal dextran treated fetal bovine serum -steroid-free-).[[File:MCF-7 Cells.jpg|thumb|[[MCF-7]] cell.|left|222x222px]][[File: ...g a positive control (standard dose-response curve), and treated only with hormone-free medium as a negative control. The bioassay ends on day 6 (late exponen ...