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...M) for Quantitative and Sensitive Live-Cell Imaging: A Novel Technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins |journal=Molecular Pla ...ey the same fluorescence emission statistics and the time evolution of the fluorescence emission after a pulsed excitation can be described by a monoexponential de ...

...obe spectroscopy|pump-probe]] method with the principle of [[laser-induced fluorescence]].{{cn|date=June 2023}} == Fluorescence measurement principle == ...

...tical sectioning of pollen.jpg|thumb|upright=1.4|(a) Optically sectioned [[fluorescence]] images of a [[pollen]] grain. (b) Combined image. (c) Combined image of a ...rent techniques for optical sectioning are used and several [[microscopy]] techniques are specifically designed to improve the quality of optical sectioning. ...

...le:Multicolor fluorescence image of living HeLa cells.jpg|thumb|Multicolor fluorescence image of living HeLa cells|alt=|250x250px]] '''Fluorescence imaging''' is a type of non-invasive imaging technique that can help visual ...

...d|magnetic]] fields used to confine the ions. Depending on the confinement techniques and parameters, as well as the number of ions in the trap, these can be 1-, Trapping techniques include variations on the [[Paul trap]] and [[Penning trap]], where the for ...

...ts of it to dissociate in a predictable manner from AT rich regions (red). fluorescence emissions from this partially denatured molecule is measured with photospec ...ese channels, denaturation profile of these molecules can be observed by [[fluorescence microscopy]].<ref name="reisner" /> ...

...itation methods, the excited molecule relaxes back to the ground state via fluorescence emission.|right|upright=2]] ...0.1111/j.1751-1097.1996.tb03116.x|title=Two‐Color Two‐Photon Excitation of Fluorescence| journal=Photochemistry and Photobiology| volume=64|issue=4|pages=632–635|y ...

...ance energy transfer|FRET]], and as a fluorescent [[Fluorophore|probe]] in fluorescence [[microscopy]] and [[X-ray crystallography]].<ref name=":1" /> [[File:TNP B ...wata |first2=Tomoko |last3=Yamada |first3=Kazuhiro |date=March 1999 |title=Fluorescence Changes of a Label Attached near the Myosin Active Site on Nucleotide Bindi ...

...Gerritsen. "Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images." Optics Express 20.12 (2012): 12729-12741.</ref ..."Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data." J. Microsc. 213 (2004): 1-5.</ref> ...

{{Short description|Fluorescence imaging technology}} ...the possibility of applying three-photon excitation microscopy to scanning fluorescence microscopy, paving the way for later applications of 3PEF. ...

...on. Compared to two-photon microscopy, three-photon microscopy reduces the fluorescence away from the focal plane by <math>1/z^4</math>, which is much faster than ...ki |first6=I |last7=Lakowicz |first7=J R |title=Three-photon excitation in fluorescence microscopy |journal=Journal of Biomedical Optics |date=1 Jan 1996 |volume=1 ...

...optics | non-linear optical technique]], which is used to detect transient fluorescence with a very high time-resolution. More recently, the term has been used to Fluorescence upconversion (FU) is a technique, which relies on the use of temporally sho ...

#to detect correlations between spectra of different techniques, for example [[near infrared spectroscopy]] (NIR) and [[Raman spectroscopy] ...y fluorescence|XRF]], [[UV/VIS spectroscopy]], [[fluorescence spectroscopy|fluorescence]], [[infrared spectroscopy|infrared]], and [[Raman spectroscopy|Raman]] spe ...

...n can be measured since the resonant RF field induces a marked decrease in fluorescence intensity. There may be many such transitions, and their characteristics as ...rough these frequencies results in two characteristic dips in the observed fluorescence, the separation between which enables determination of the strength of the ...

...istinguishable states, either real on-/off-states or states with different fluorescence intensities. In mathematical terms SOFI-imaging relies on the calculation o ...l value intensities are a measure of the brightness and correlation of the fluorescence signal. Mathematically, the ''n''-th order cumulant is related to the ''n'' ...

...ier |last3=Stelzer |first3=Ernst H. K. |title=csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution be ...ast3=Stemmer |first3=A. |title=Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation |journal=Optics Letters |date=1 June 2001 ...

...encies that are characteristic to each element.<ref>{{cite web|title=X-Ray Fluorescence (XRF): Understanding Characteristic X-Rays|url=http://www.amptek.com/pdf/ch ...iffraction |url=https://serc.carleton.edu/research_education/geochemsheets/techniques/SXD.html |website=Geochemical Instrumentation and Analysis |publisher=Carle ...

...first1=E. |last2=Queralt |first2=I. |last3=Almeida |first3=E. |title=X-ray fluorescence spectrometry for environmental analysis: Basic principles, instrumentation, ...ring. The method is called HERFD, which stands for High Energy Resolution Fluorescence Detection. The collection method is unique in that, after a collection of a ...

...l extract in alcohol shown under white light (above) and UV light inducing fluorescence (below).]] [[File:Chlorophyll Fluorescence.jpg|thumb|Confocal microscope images of a tomato leaf from ''Solanum lycope ...

{{short description|Fluorescence microscopy imaging method}} ...P. Giriajan |author3=M. D. Mason |title= Ultra-high resolution imaging by Fluorescence Photoactivation Localization Microscopy |journal= Biophysical Journal |volu ...