File:Localization of a fluorophore using MINFLUX.png
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Size of this preview: 800 × 461 pixels. Other resolutions: 320 × 185 pixels | 640 × 369 pixels | 1,024 × 591 pixels | 1,280 × 738 pixels | 2,560 × 1,477 pixels | 3,672 × 2,118 pixels.
Original file (3,672 × 2,118 pixels, file size: 1.73 MB, MIME type: image/png)
Summary
| Description |
English: MINFLUX localizes an active fluorescent molecule using a probing scheme. The doughnut-shaped excitation beam is positioned at different points at the periphery and in the middle of a probing area L. The active molecule is excited at each position, and photon fluxes are recorded. MINFLUX uses the flux patterns of these recordings to recenter the excitation beam closer to the active molecule and then performs another probing iteration. With each iteration, the probing area L is constricted, and the intensity zero of the excitation beam more accurately overlaps with the position of the active molecule. |
| Date | |
| Source | Own work |
| Author | AByolia |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:53, 29 January 2024 | | 3,672 × 2,118 (1.73 MB) | wikimediacommons>AByolia | Uploaded own work with UploadWizard |
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