View source for N-hydroxy-2-acetamidofluorene reductase

{{infobox enzyme
| Name       = N-hydroxy-2-acetamidofluorene reductase
| EC_number  = 1.7.1.12
| CAS_number = 99890-08-1
| GO_code    = 0047137
| image      =
| width      = 
| caption    =  
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In [[enzymology]], a '''N-hydroxy-2-acetamidofluorene reductase''' ({{EC number|1.7.1.12}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:2-acetamidofluorene + NAD(P)+ + H<sub>2</sub>O <math>\rightleftharpoons</math> N-hydroxy-2-acetamidofluorene + NAD(P)H + H<sup>+</sup>

The 4 [[substrate (biochemistry)|substrates]] of this enzyme are [[2-acetamidofluorene]], [[nicotinamide adenine dinucleotide|NAD<sup>+</sup>]], [[nicotinamide adenine dinucleotide phosphate|NADP<sup>+</sup>]], and [[water|H<sub>2</sub>O]], whereas its 4 [[product (chemistry)|products]] are [[N-hydroxy-2-acetamidofluorene]], [[nicotinamide adenine dinucleotide|NADH]], [[nicotinamide adenine dinucleotide phosphate|NADPH]], and [[hydrogen ion|H<sup>+</sup>]].

This enzyme belongs to the family of [[oxidoreductase]]s, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor.  The [[List of enzymes|systematic name]] of this enzyme class is '''2-acetamidofluorene:NAD(P)+ oxidoreductase'''. Other names in common use include '''N-hydroxy-2-acetylaminofluorene reductase''', and '''NAD(P)H:N-hydroxy-2-acetamidofluorene N-oxidoreductase'''.

==References==
{{reflist|1}}
* {{cite journal |vauthors=Gutmann HR, Erickson RR | date = 1969 | title = The conversion of the carcinogen N-hydroxy-2-fluorenylacetamide to o-amidophenols by rat liver in vitro. An inducible enzymatic reaction | journal = J. Biol. Chem.  | volume = 244 | pages = 1729&ndash;40  | pmid = 5780838 | issue = 7 }}
* {{cite journal |vauthors=Kitamura S, Tatsumi K | date = 1985 | title = Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol | journal = Biochem. Biophys. Res. Commun.  | volume = 133 | pages = 67&ndash;74  | pmid = 4074379 | doi = 10.1016/0006-291X(85)91842-X | issue = 1 }}

{{Nitrogenous donor oxidoreductases}}
{{Enzymes}}
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[[Category:EC 1.7.1]]
[[Category:NADPH-dependent enzymes]]
[[Category:NADH-dependent enzymes]]
[[Category:Enzymes of unknown structure]]


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