View source for Lipid A phosphoethanolamine transferase

{{Short description|Enzyme}}
{{enzyme
| Name =Lipid A phosphoethanolamine transferase
| EC_number = 2.7.8.43
| CAS_number = 
| GO_code = 
| image = 
| width = 
| caption = 
}}
{{Pfam box
|InterPro=IPR040423
|Symbol=PEA_transferase
}}
{{Pfam box
|Name=Phosphoethanolamine transferase, N-terminal
|Symbol= EptA-like_N
|Pfam=PF08019
|InterPro=IPR012549
}}
'''Lipid A phosphoethanolamine transferase''' (EC 2.7.8.43, '''lipid A PEA transferase''', ''LptA'', formerly EC 2.7.4.30) is an [[enzyme]] that modifies [[Lipid A]] by linkage to a [[phosphoethanolamine]] moiety. Doing so at some positions reduces the affinity to [[colistin]] and related [[polymyxins]], resulting in reduced activity of the antimicrobial. This type of resistance is known as target modification.<ref name="HinchliffeYang2017">{{cite journal | vauthors = Hinchliffe P, Yang QE, Portal E, Young T, Li H, Tooke CL, Carvalho MJ, Paterson NG, Brem J, Niumsup PR, Tansawai U, Lei L, Li M, Shen Z, Wang Y, Schofield CJ, Mulholland AJ, Shen J, Fey N, Walsh TR, Spencer J | title = Insights into the Mechanistic Basis of Plasmid-Mediated Colistin Resistance from Crystal Structures of the Catalytic Domain of MCR-1 | journal = Scientific Reports | volume = 7 | pages = 39392 | date = January 2017 | pmid = 28059088 | pmc = 5216409 | doi = 10.1038/srep39392 | bibcode = 2017NatSR...739392H }}</ref> This type of enzyme is of special medical note, as it offers resistance to a [[drug of last resort|last-resort antibiotic]].<ref name=Liu15>{{cite journal | vauthors = Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J | title = Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study | journal = The Lancet. Infectious Diseases | volume = 16 | issue = 2 | pages = 161–8 | date = February 2016 | pmid = 26603172 | doi = 10.1016/S1473-3099(15)00424-7 }}</ref> The modifications also provide cross-resistance to host immunity factors, specifically [[antimicrobial peptide]]s and [[lysozyme]].<ref>{{cite journal | vauthors = Napier BA, Burd EM, Satola SW, Cagle SM, Ray SM, McGann P, Pohl J, Lesho EP, Weiss DS | title = Clinical Use of Colistin Induces Cross-Resistance to Host Antimicrobials in Acinetobacter baumannii | journal = mBio | volume = 4 | issue = 3 | pages = e00021–13–e00021–13 | date = 21 May 2013 | pmid = 23695834 | doi = 10.1128/mBio.00021-13 | pmc=3663567}}</ref><ref name=pmid37094804>{{cite journal |last1=Jangir |first1=Pramod K |last2=Ogunlana |first2=Lois |last3=Szili |first3=Petra |last4=Czikkely |first4=Marton |last5=Shaw |first5=Liam P |last6=Stevens |first6=Emily J |last7=Yang |first7=Yu |last8=Yang |first8=Qiue |last9=Wang |first9=Yang |last10=Pál |first10=Csaba |last11=Walsh |first11=Timothy R |last12=MacLean |first12=Craig R |title=The evolution of colistin resistance increases bacterial resistance to host antimicrobial peptides and virulence |journal=eLife |date=25 April 2023 |volume=12 |pages=e84395 |doi=10.7554/eLife.84395|pmid=37094804 |pmc=10129329 |doi-access=free }}</ref> EC 2.7.8.43 catalyzes one of the following three reactions:<ref>{{cite web |title=ENZYME - 2.7.8.43 lipid A phosphoethanolamine transferase |url=https://enzyme.expasy.org/EC/2.7.8.43 |website=enzyme.expasy.org}}</ref>
* a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H(+) + lipid A (E. coli) <math>\rightleftharpoons</math> a 1,2-diacyl-sn-glycerol + lipid A hexaacyl 1-(2-aminoethyl diphosphate)
* a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H(+) + lipid A (E. coli) <math>\rightleftharpoons</math> a 1,2-diacyl-sn-glycerol + lipid A 4'-(2-aminoethyl diphosphate) (E. coli)
* a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H(+) + lipid A hexaacyl 1-(2-aminoethyl diphosphate) <math>\rightleftharpoons</math> a 1,2-diacyl-sn-glycerol + lipid A 1,4'-bis(2-aminoethyl diphosphate)

Enzyme databases may list a very long list of synonyms for this enzyme. Many of these names, such as ''[[mcr-1]]'', do not refer to this type of enzyme in general, but only to a specific member of the family.<ref name="brenda">{{cite web |title=2.7.8.43: lipid A phosphoethanolamine transferase - BRENDA Enzyme Database |url=https://www.brenda-enzymes.org/all_enzymes.php?ecno=2.7.8.43&table=Systematic_Name |website=www.brenda-enzymes.org}}</ref> There are many non-mobile (chromosomal) versions of this enzyme scattered all around the evolutionary tree, but ''mcr-1'' was notable because it was found on a [[plasmid]], therefore capable of [[horizontal gene transfer]].<ref name=pmid29636432>{{cite journal | vauthors = Xu Y, Wei W, Lei S, Lin J, Srinivas S, Feng Y | title = An Evolutionarily Conserved Mechanism for Intrinsic and Transferable Polymyxin Resistance | journal = mBio | volume = 9 | issue = 2 | date = April 2018 | pmid = 29636432 | pmc = 5893884 | doi = 10.1128/mBio.02317-17 }}</ref> Only one family of protein is currently known to perform the activity described by the EC number.

== Structure ==
The enzyme is composed of two domains. The N-terminal part (about 1/3 of the length) is a transmembrane domain, while the rest is catalytic. Both domains contribute to the phosphoethanolamine substrate cavity. The C-terminal domain binds zinc as a cofactor.<ref name=pmid29636432/>

== Function ==
Polymyxins and other cationic antimicrobial peptides attach to the LPS cell walls of bacteria by virtue of the highly negatively-charged groups in LPS such as Lipid A and Kdo. Modification of LPS with positively-charged PEA shields these sites from binding.<ref>{{cite journal |last1=Anaya-López |first1=José Luis |last2=López-Meza |first2=Joel Edmundo |last3=Ochoa-Zarzosa |first3=Alejandra |title=Bacterial resistance to cationic antimicrobial peptides |journal=Critical Reviews in Microbiology |date=May 2013 |volume=39 |issue=2 |pages=180–195 |doi=10.3109/1040841x.2012.699025|pmid=22799636 |s2cid=35828720 }}</ref>

Not all members of this family perform the same reaction, contrary to the EC classification framework. For example, ''E. coli'' naturally has three related genes all from this family, ''EptA'' through ''C'', all with different preferences for where to attach PEA. Addition of PEA can happen on Lipid A (this EC entry), on [[3-Deoxy-D-manno-oct-2-ulosonic acid|Kdo]] ([[EC 2.7.8.42]]), or on Heptose 1 (no EC number), the latter two being parts of the [[core oligosaccharide]]. In the case of ''EptC'', addition of PEA to Heptose compacts the LPS by forming a network of [[hydrogen bond]]s.<ref name="EptC">{{cite journal |last1=Salazar |first1=Javier |last2=Alarcón |first2=Mackarenna |last3=Huerta |first3=Jaime |last4=Navarro |first4=Belén |last5=Aguayo |first5=Daniel |title=Phosphoethanolamine addition to the Heptose I of the Lipopolysaccharide modifies the inner core structure and has an impact on the binding of Polymyxin B to the Escherichia coli outer membrane |journal=Archives of Biochemistry and Biophysics |date=April 2017 |volume=620 |pages=28–34 |doi=10.1016/j.abb.2017.03.008|pmid=28342805 }}</ref>

== Regulation ==
In chromosomal versions of this enzyme, the gene is regulated by a [[two-component regulatory system]] termed ''PmrAB'' or ''BasRS''. The ''PmrA'' or ''BasS'' is the [[histidine kinase]] sensor, which activates the DNA-binding [[response regulator]] ''BasR'' or ''PmrB''. The sensor triggers in a variety of dangerous situations, such as metal ions and being ingested by a [[phagocyte]], helping the bacterium build a stronger cell wall to survive. The ''PhoPQ'' system, which detects similar situations and the presence of antimicrobial peptides, can also cross-trigger ''PmrA'' via a ''PmrD'' connector. Antibiotic resistance can occur when this system, or its upstream signals, mutates to become constitutively active.<ref name=pmid28275006>{{cite journal |last1=Poirel |first1=L |last2=Jayol |first2=A |last3=Nordmann |first3=P |title=Polymyxins: Antibacterial Activity, Susceptibility Testing, and Resistance Mechanisms Encoded by Plasmids or Chromosomes. |journal=Clinical Microbiology Reviews |date=April 2017 |volume=30 |issue=2 |pages=557–596 |doi=10.1128/CMR.00064-16 |pmid=28275006|pmc=5355641}}</ref>

In plasmid versions, the gene is simply constitutively activated by an upstream promoter.<ref name=pmid28275006/> The extra metabolic resources diverted means that the resistant trait is disadvantageous in environments without antibiotic or antimicrobial peptide threats, specifically by about 3%.<ref name=pmid37094804/>

== See also ==
* [[MCR-1]]

== References ==
{{reflist}}

{{Kinases}}
{{Enzymes}}
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[[Category:EC 2.7.8]]